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lexa dna-binding domain  (Millipore)

 
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    Structured Review

    Millipore lexa dna-binding domain
    (A) Schematic showing ZAR1 domain truncation boundaries. Subdomains of the central nucleotide-binding region are labeled as described by Wang et al [ , ]: NBD, nucleotide-binding domain; HD1, helical domain 1; and WHD, winged helix domain. (B) Confirmation of expression for each of the three ZAR1 constructs shown in panel A. A western blot of yeast cell lysates probed with serum raised against the <t>LexA</t> <t>DNA-binding</t> domain (top) is compared with Ponceau S staining of the total protein in each lane (bottom). (C) Interactions between ZAR1 bait constructs and 11 PBL preys were assessed in the absence and presence of HopZ1a and/or ZED1 alleles integrated at the ho locus of strains EGY48 ( MAT α ) or RFY206 ( MAT A ), corresponding to Y2H (interaction scheme A), Y3H (interaction schemes B), and Y4H (interaction scheme C) assays (see ). The prey array layout shown at right represents PBL interactions with ZAR1 ΔCC in the presence of both ZED1 and HopZ1a wt . The colours of the labels at each array position (white or black) are determined by the relative interaction strength (see ; Materials and Methods).
    Lexa Dna Binding Domain, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lexa dna-binding domain/product/Millipore
    Average 90 stars, based on 1 article reviews
    lexa dna-binding domain - by Bioz Stars, 2026-02
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    Images

    1) Product Images from "Perturbations of the ZED1 pseudokinase activate plant immunity"

    Article Title: Perturbations of the ZED1 pseudokinase activate plant immunity

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1007900

    (A) Schematic showing ZAR1 domain truncation boundaries. Subdomains of the central nucleotide-binding region are labeled as described by Wang et al [ , ]: NBD, nucleotide-binding domain; HD1, helical domain 1; and WHD, winged helix domain. (B) Confirmation of expression for each of the three ZAR1 constructs shown in panel A. A western blot of yeast cell lysates probed with serum raised against the LexA DNA-binding domain (top) is compared with Ponceau S staining of the total protein in each lane (bottom). (C) Interactions between ZAR1 bait constructs and 11 PBL preys were assessed in the absence and presence of HopZ1a and/or ZED1 alleles integrated at the ho locus of strains EGY48 ( MAT α ) or RFY206 ( MAT A ), corresponding to Y2H (interaction scheme A), Y3H (interaction schemes B), and Y4H (interaction scheme C) assays (see ). The prey array layout shown at right represents PBL interactions with ZAR1 ΔCC in the presence of both ZED1 and HopZ1a wt . The colours of the labels at each array position (white or black) are determined by the relative interaction strength (see ; Materials and Methods).
    Figure Legend Snippet: (A) Schematic showing ZAR1 domain truncation boundaries. Subdomains of the central nucleotide-binding region are labeled as described by Wang et al [ , ]: NBD, nucleotide-binding domain; HD1, helical domain 1; and WHD, winged helix domain. (B) Confirmation of expression for each of the three ZAR1 constructs shown in panel A. A western blot of yeast cell lysates probed with serum raised against the LexA DNA-binding domain (top) is compared with Ponceau S staining of the total protein in each lane (bottom). (C) Interactions between ZAR1 bait constructs and 11 PBL preys were assessed in the absence and presence of HopZ1a and/or ZED1 alleles integrated at the ho locus of strains EGY48 ( MAT α ) or RFY206 ( MAT A ), corresponding to Y2H (interaction scheme A), Y3H (interaction schemes B), and Y4H (interaction scheme C) assays (see ). The prey array layout shown at right represents PBL interactions with ZAR1 ΔCC in the presence of both ZED1 and HopZ1a wt . The colours of the labels at each array position (white or black) are determined by the relative interaction strength (see ; Materials and Methods).

    Techniques Used: Binding Assay, Labeling, Expressing, Construct, Western Blot, Staining



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    (A) Schematic showing ZAR1 domain truncation boundaries. Subdomains of the central nucleotide-binding region are labeled as described by Wang et al [ , ]: NBD, nucleotide-binding domain; HD1, helical domain 1; and WHD, winged helix domain. (B) Confirmation of expression for each of the three ZAR1 constructs shown in panel A. A western blot of yeast cell lysates probed with serum raised against the <t>LexA</t> <t>DNA-binding</t> domain (top) is compared with Ponceau S staining of the total protein in each lane (bottom). (C) Interactions between ZAR1 bait constructs and 11 PBL preys were assessed in the absence and presence of HopZ1a and/or ZED1 alleles integrated at the ho locus of strains EGY48 ( MAT α ) or RFY206 ( MAT A ), corresponding to Y2H (interaction scheme A), Y3H (interaction schemes B), and Y4H (interaction scheme C) assays (see ). The prey array layout shown at right represents PBL interactions with ZAR1 ΔCC in the presence of both ZED1 and HopZ1a wt . The colours of the labels at each array position (white or black) are determined by the relative interaction strength (see ; Materials and Methods).
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    (A) Schematic showing ZAR1 domain truncation boundaries. Subdomains of the central nucleotide-binding region are labeled as described by Wang et al [ , ]: NBD, nucleotide-binding domain; HD1, helical domain 1; and WHD, winged helix domain. (B) Confirmation of expression for each of the three ZAR1 constructs shown in panel A. A western blot of yeast cell lysates probed with serum raised against the <t>LexA</t> <t>DNA-binding</t> domain (top) is compared with Ponceau S staining of the total protein in each lane (bottom). (C) Interactions between ZAR1 bait constructs and 11 PBL preys were assessed in the absence and presence of HopZ1a and/or ZED1 alleles integrated at the ho locus of strains EGY48 ( MAT α ) or RFY206 ( MAT A ), corresponding to Y2H (interaction scheme A), Y3H (interaction schemes B), and Y4H (interaction scheme C) assays (see ). The prey array layout shown at right represents PBL interactions with ZAR1 ΔCC in the presence of both ZED1 and HopZ1a wt . The colours of the labels at each array position (white or black) are determined by the relative interaction strength (see ; Materials and Methods).
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    (A) Schematic showing ZAR1 domain truncation boundaries. Subdomains of the central nucleotide-binding region are labeled as described by Wang et al [ , ]: NBD, nucleotide-binding domain; HD1, helical domain 1; and WHD, winged helix domain. (B) Confirmation of expression for each of the three ZAR1 constructs shown in panel A. A western blot of yeast cell lysates probed with serum raised against the <t>LexA</t> <t>DNA-binding</t> domain (top) is compared with Ponceau S staining of the total protein in each lane (bottom). (C) Interactions between ZAR1 bait constructs and 11 PBL preys were assessed in the absence and presence of HopZ1a and/or ZED1 alleles integrated at the ho locus of strains EGY48 ( MAT α ) or RFY206 ( MAT A ), corresponding to Y2H (interaction scheme A), Y3H (interaction schemes B), and Y4H (interaction scheme C) assays (see ). The prey array layout shown at right represents PBL interactions with ZAR1 ΔCC in the presence of both ZED1 and HopZ1a wt . The colours of the labels at each array position (white or black) are determined by the relative interaction strength (see ; Materials and Methods).
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    (A) Schematic showing ZAR1 domain truncation boundaries. Subdomains of the central nucleotide-binding region are labeled as described by Wang et al [ , ]: NBD, nucleotide-binding domain; HD1, helical domain 1; and WHD, winged helix domain. (B) Confirmation of expression for each of the three ZAR1 constructs shown in panel A. A western blot of yeast cell lysates probed with serum raised against the <t>LexA</t> <t>DNA-binding</t> domain (top) is compared with Ponceau S staining of the total protein in each lane (bottom). (C) Interactions between ZAR1 bait constructs and 11 PBL preys were assessed in the absence and presence of HopZ1a and/or ZED1 alleles integrated at the ho locus of strains EGY48 ( MAT α ) or RFY206 ( MAT A ), corresponding to Y2H (interaction scheme A), Y3H (interaction schemes B), and Y4H (interaction scheme C) assays (see ). The prey array layout shown at right represents PBL interactions with ZAR1 ΔCC in the presence of both ZED1 and HopZ1a wt . The colours of the labels at each array position (white or black) are determined by the relative interaction strength (see ; Materials and Methods).
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    Image Search Results


    (A) Schematic showing ZAR1 domain truncation boundaries. Subdomains of the central nucleotide-binding region are labeled as described by Wang et al [ , ]: NBD, nucleotide-binding domain; HD1, helical domain 1; and WHD, winged helix domain. (B) Confirmation of expression for each of the three ZAR1 constructs shown in panel A. A western blot of yeast cell lysates probed with serum raised against the LexA DNA-binding domain (top) is compared with Ponceau S staining of the total protein in each lane (bottom). (C) Interactions between ZAR1 bait constructs and 11 PBL preys were assessed in the absence and presence of HopZ1a and/or ZED1 alleles integrated at the ho locus of strains EGY48 ( MAT α ) or RFY206 ( MAT A ), corresponding to Y2H (interaction scheme A), Y3H (interaction schemes B), and Y4H (interaction scheme C) assays (see ). The prey array layout shown at right represents PBL interactions with ZAR1 ΔCC in the presence of both ZED1 and HopZ1a wt . The colours of the labels at each array position (white or black) are determined by the relative interaction strength (see ; Materials and Methods).

    Journal: PLoS Pathogens

    Article Title: Perturbations of the ZED1 pseudokinase activate plant immunity

    doi: 10.1371/journal.ppat.1007900

    Figure Lengend Snippet: (A) Schematic showing ZAR1 domain truncation boundaries. Subdomains of the central nucleotide-binding region are labeled as described by Wang et al [ , ]: NBD, nucleotide-binding domain; HD1, helical domain 1; and WHD, winged helix domain. (B) Confirmation of expression for each of the three ZAR1 constructs shown in panel A. A western blot of yeast cell lysates probed with serum raised against the LexA DNA-binding domain (top) is compared with Ponceau S staining of the total protein in each lane (bottom). (C) Interactions between ZAR1 bait constructs and 11 PBL preys were assessed in the absence and presence of HopZ1a and/or ZED1 alleles integrated at the ho locus of strains EGY48 ( MAT α ) or RFY206 ( MAT A ), corresponding to Y2H (interaction scheme A), Y3H (interaction schemes B), and Y4H (interaction scheme C) assays (see ). The prey array layout shown at right represents PBL interactions with ZAR1 ΔCC in the presence of both ZED1 and HopZ1a wt . The colours of the labels at each array position (white or black) are determined by the relative interaction strength (see ; Materials and Methods).

    Article Snippet: Simultaneous expression of bait-fusion, prey-fusion and integrated FLAG-tagged effectors in diploid cells carrying bait, prey and reporter plasmids is demonstrated in similar western blots shown in panel C of . Primary antibodies against HA (Roche), the LexA DNA-binding domain (Sigma) and FLAG (Sigma), as well as HRP-conjugated secondary antibodies (Cell Signalling) were all applied as 1:10,000 dilutions in TBST (50 mM Tris-Cl, pH 7.5; 150 mM NaCl; 0.05% Tween-20) with 3% powdered skim milk.

    Techniques: Binding Assay, Labeling, Expressing, Construct, Western Blot, Staining